Workshop Description

This workshop will introduce the basic principles of DNA methylation and briefly describe available methods for DNA methylation assessment, focusing on bisulfite sequencing (BSseq). We will cover the principles of BSseq preprocessing, alignment and methylation calling, differential methylation analysis, annotation and visualization. Attendees will  have the opportunity to work on real DNA methylation data provided by the instructor and run the analysis on Hoffman2 and locally. Attendees need to have a basic knowledge in UNIX commands and R programming for this workshop. No previous experience with NGS or DNA methylation analysis is necessary. 

TBA

TBA

TBA

Technical Requirements

  • Attendees are required to have a Hoffman2 account. To apply for an account, click here. UCLA participants who lack a faculty sponsor and non-UCLA participants may apply for a temporary Hoffman2 account, requesting sponsorship from Collaboratory Workshops.
  • We strongly encourage attendees to bring a laptop capable of accessing UCLA’s WiFi.
  • It is recommended for attendees to have R (https://cran.r-project.org/mirrors.html)/Rstudio (https://www.rstudio.com/) installed locally (on their own laptop).

Instructor

Dr. Anela Tosevska is currently a postdoctoral fellow in the lab of Dr. Matteo Pellegrini at UCLA. She completed her master degree in Pharmacy at Ss. Cyril and Methodius University of Skopje. Before joining UCLA, she earned a PhD in Life Sciences from the University of Vienna, working as a part of the research platform Active Aging. Dr. Tosevska is interested in the role of DNA methylation in aging and age-associated disease progression. She is also working with circulating cell-free nucleic acids from liquid biopsies, trying to understand their function and diagnostic potential in health and disease. Email: atosevska@ucla.edu

Workshop Details

Prerequisites: W1 (Intro to UNIX) and W3 (Intro to R) or equivalent experience with UNIX and R programming.   Recommended: W2 (Using NGS Analysis tools)

Length: 3 days, 3 hrs per day
Level: Introductory
Location: Collaboratory Classroom  (Boyer Hall, 529)
Seats Available: 15

Winter 2020 Dates

February 25, 26, and 27, 2020
1:30 PM to 4:30 PM

REGISTRATION DEADLINE IS: FEB. 19, 2020!