Workshop Description

RNA-seq II aims to provide tools for analysis of gene expression data from read counts to biology using R. To facilitate learning, the workshop will use a real case study based approach appropriate for Illumina read data (same as RNA-seq I).

RNA-Seq II (Alice Mouton, Ph.D and Yerbol Kurmangaliyev, Ph.D):

From gene expression to biology (gene expression analysis):

  • Normalization of gene expression
  • Exploratory analysis of RNA-Seq data
  • Differential gene expression analysis
  • Gene set and pathway enrichment analysis
  • Visualization of gene expression

Technical Requirements

Attendees are required to have pre-installed R and Rstudio. Other details will be sent to registered attendees by email prior to workshop.

Instructor

Dr. Alice Mouton is an Assistant Project Scientist in Robert Wayne laboratory in the department of Ecology and Evolutionary Biology. Her current researches focus on applying gene expression methodologies (RNA-seq and RRBS) to study non model organisms (bobcat, island fox) in a context of conservation.

Dr. Yerbol Kurmangaliev is currently a postdoctoral researcher in the laboratory of Professor S. Lawrence Zipursky. He earned a M.S. degree in Biochemistry at Lomonosov Moscow State University in 2006. He earned a Ph.D. degree in Bioinformatics at Kharkevich Institute for information transmission problems of the Russian Academy of Sciences in 2011 under the supervision of Professor Mikhail S. Gelfand. Before joining to UCLA he was a postdoctoral researcher in the laboratory of Professor Sergey V. Nuzhdin at USC. His previous research interests have been primarily focused on genetic basis of gene expression.

Workshop Details

Prerequisites: W3
Recommended: W5a
Length: 3 days, 2-3hrs per day
Level: Intermediate
Location: Collaboratory Classroom  (Boyer Hall, 529)
Seats Available: 28

Winter Dates

Feb. 6 – 8, 9:30 – 12:30pm