Workshop Description

This workshop will cover the basis of Next-Gen Sequencing Library Preparation for Illumina Sequencers. Different Library Preparation Techniques (DNA-seq, ChIP-seq, RNA-seq, Methyl-seq) are explained the first and second day in class. The third day, Quality Control steps of the starting input material and final libraries are performed in the Lab (TapeStation). Purification from Primer Dimers (using AMPure beads) and Library Submission is also performed on the same day. The third day is optional, sign-up is required.

Day 1 - Library Prep 101, focus on DNA

  • Theory behind Next Generation Sequencing Library Preparation
  • DNA Library Preps (WGS and WES)
  • Other Library Preps:
    • DNA Methylation
    • ChIP-seq
    • ATAC-seq

Day 2 - Focus on RNA Library Prep

  • RNA Library Preps:
    • mRNAseq
    • total RNA
    • small RNA
    • single-cell seq

Day 3 – Session A or session B

(Pellegrini Lab – TLSB, room 4021)

  • Quality control of RNA samples
  • Quality control of final libraries
  • AMPure beads Purification and Size Selection of Sonicated DNA

Technical Requirements

Day 2 will take place in a wet lab. Attendees will need the following prior to the start of class:

Instructor

Dr. Marco Morselli is currently a post-doc in the Lab of Professor Matteo Pellegrini. He received his bachelor and master degrees in Molecular Biotechnology at the University of Parma, Italy. He earned his PhD at UCLA focusing on the crosstalk between histone modifications and DNA methylation. Outside of the lab, Marco enjoys cooking, running, cycling and playing soccer.

Email: mmorselli@ucla.edu

Workshop Details

Prerequisites: Lab Safety Training
Length: 2 days, 3hrs per day
Level: Introductory
Location: Collaboratory Classroom  (Boyer Hall, 529)
Seats Available: 28

Spring Dates

Day 1 & 2:
May 15 & 16,  1pm – 4pm
Day 3 (optional):
May 17 Session A or B

  • Session A, 9-12pm
  • Session B, 1-4pm