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X-WR-CALDESC:Events for Institute for Quantitative and Computational Biosciences
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DTSTART;TZID=America/Los_Angeles:20231103T130000
DTEND;TZID=America/Los_Angeles:20231103T133000
DTSTAMP:20231103T205708Z
CREATED:20231019T182813Z
LAST-MODIFIED:20231103T205708Z
UID:25904-1699016400-1699018200@qcb.ucla.edu
SUMMARY:Research-in-Progress (RIP) Seminar: Amantha O'Keeffe (Park)\, Graduate Student in Chemical and Biomolecular Engineering
DESCRIPTION:TITLE: “Quantification of Absolute Metabolite Concentrations in T cells by Shotgun Metabolomics.” \nABSTRACT: Quantitative understanding of immunometabolism underlies improving immune functions and developing successful immunotherapies. Kinetic and thermodynamic laws rely on absolute\, not relative\, metabolite concentrations to map metabolism. However\, until now\, comprehensive absolute metabolite quantification has been inaccessible due to the need for iterative analytical procedures involving internal standards. Here we developed a simple technique to facilitate absolute metabolite quantitation. Shotgun metabolomics leverages the known absolute metabolite concentrations of model systems and 13C labeling to distinguish metabolites from two different cell types in a single extraction sample. We cultured T cells in unlabeled media and E. coli or epithelial cells in 13C-labeled media and extracted their metabolites simultaneously. Using LC-MS and known concentrations of 13C-labeled metabolites as internal standards\, we quantified ~80 metabolites en masse in human T cells. \n\nhttps://wp-misc.lifesci.ucla.edu/qcb/wp-content/uploads/sites/14/2023/10/Samantha-OKeeffe.mp4
URL:https://qcb.ucla.edu/event/research-in-progress-rip-seminar-amantha-okeeffe-park-graduate-student-in-chemical-and-biomolecular-engineering/
LOCATION:ZOOM\, CA\, United States
CATEGORIES:QCBio Seminar Series
ATTACH;FMTTYPE=image/jpeg:https://qcb.ucla.edu/wp-content/uploads/sites/14/2023/10/samantha.jpg
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DTSTART;TZID=America/Los_Angeles:20231103T133000
DTEND;TZID=America/Los_Angeles:20231103T140000
DTSTAMP:20231103T210314Z
CREATED:20231027T181948Z
LAST-MODIFIED:20231103T210314Z
UID:25967-1699018200-1699020000@qcb.ucla.edu
SUMMARY:Research-in-Progress (RIP) Seminar: Alexis Weber (de la Torre-Ubieta and Geschwind)\, Graduate Student in Human Genetics
DESCRIPTION:TITLE: “Defining molecular dysregulation in Down Syndrome neocortex and neural progenitor cells.” \nABSTRACT: Down syndrome (DS) is the most common form of genetic\, intellectual disability\, which occurs 1 in 700 newborns and presents in patients as cognitive deficits\, particularly diminished in learning\, memory\, and language development.1\,2\,3 DS symptoms result from impaired cortical development\, which is demonstrated\, in contrast to neurotypical (NTD) brains\, by postnatally reduced whole brain weight\, volume and surface area\, lower numbers of progenitors\, excitatory neurons and oligodendrocytes\, increased numbers of astrocytes\, interneurons and microglia\, and altered neuronal morphology\, maturation\, and migration.3–10 These DS neuropathologies result through some means from the triplication of human chromosome 21 (hsa21) or trisomy 21 (T21). However\, the way in which T21 confers DS pathology and inhibits cortical development remains unclear. I hypothesize that increased hsa21 gene dosage alters global gene expression in neural progenitors\, changing neural cell fate specification and differentiation. By single nuclei Multiome sequencing\, T21 neocortices demonstrate a disproportionate increase in progenitors\, interneurons and oligodendrocyte precursor cells and decrease in excitatory neurons\, contrast to neurotypical (NT) donors. Furthermore\, T21 neocortices show cell-specific differential expression of critical neurodevelopmental genes and transcription factors. These data support potential\, cell-specific mechanisms of gene dysregulation during T21 neurodevelopment. \nhttps://wp-misc.lifesci.ucla.edu/qcb/wp-content/uploads/sites/14/2023/10/Alexis-Weber.mp4
URL:https://qcb.ucla.edu/event/research-in-progress-rip-seminar-alexis-weber-de-la-torre-ubieta-and-geschwind-graduate-student-in-human-genetics/
LOCATION:ZOOM\, CA\, United States
CATEGORIES:QCBio Seminar Series
ATTACH;FMTTYPE=image/png:https://qcb.ucla.edu/wp-content/uploads/sites/14/2023/10/Alexis-Weber.png
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