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DTSTART;TZID=America/Los_Angeles:20210724T140000
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DTSTAMP:20260518T041655
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UID:18460-1627135200-1627137000@qcb.ucla.edu
SUMMARY:QCBio Research Seminar in collaboration with B.I.G. Summer: Harold Pimentel
DESCRIPTION:TITLE: “Model driven design and analysis of quantitative phenotype screens” \nABSTRACT: Increasingly\, CRISPR screens are coupled with flow cytometry (FACS) to sort cells and quantify the \nimpact of genetic perturbations on a continuous phenotype. While FACS provides much more \nquantitative information than simple survival screens\, it introduces a number of experimental and \nstatistical challenges. Furthermore\, as experimentalists push these screens in limited primary cells \nor in vivo\, they lack principled guidelines on how experimental parameters including multiplicity of \ninfection\, guide RNA coverage\, or FACS bin cutoffs affect statistical power. We present models for \nboth experimental design and inference of gene regulation in these screens. We show that \ncommonly used parameters are far from optimal and screens can be performed with a 20 times \nreduction in cells at comparable accuracy. Our inference procedure models biological replicates\, \ninfers the latent protein distribution\, and infers experimental parameters. Together these analyses \nprovide a holistic framework for designing and analyzing highly parallel FACS screens. \n\nhttps://qcb.ucla.edu/wp-content/uploads/sites/14/2021/07/Harold-Pimentel-edited.mp4
URL:https://qcb.ucla.edu/event/qcbio-research-seminar-in-collaboration-with-b-i-g-summer-harold-pimentel/
LOCATION:ZOOM\, CA\, United States
CATEGORIES:Research Seminars
ATTACH;FMTTYPE=image/jpeg:https://wp-misc.lifesci.ucla.edu/qcb/wp-content/uploads/sites/14/2021/07/7.23.21-Harold-Pimentel.jpg
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