W16: Library Prep for NGS

529 Boyer Hall 611 Charles E Young Dr E,, Los Angeles, CA, United States

This workshop will cover the basis of Next-Gen Sequencing Library Preparation for Illumina Sequencers. Different Library Preparation Techniques (DNA-seq, ChIP-seq, RNA-seq, Methyl-seq) are explained the first and second day in class. The third day, Quality Control steps of the starting input material and final libraries are performed in the Lab (TapeStation). Purification from Primer Dimers […]

W5a: RNA-seq I Analysis

529 Boyer Hall 611 Charles E Young Dr E,, Los Angeles, CA, United States

RNA-seq I aims to provide an introduction and the basics tools to process raw RNA-seq data on a cluster machine (Hoffman2). The workshop can serve also as a starting point to develop a gene expression project. This workshop is divided in three days that will cover major steps of processing RNA-seq. To facilitate learning, the […]

W5b: RNA-Seq II Analysis

529 Boyer Hall 611 Charles E Young Dr E,, Los Angeles, CA, United States

RNA-seq II aims to provide tools for analysis of gene expression data from read counts to biology using R. To facilitate learning, the workshop will use a real case study based approach appropriate for Illumina read data (same as RNA-seq I).

W20: Single-Cell RNA-Seq Analysis with R

529 Boyer Hall 611 Charles E Young Dr E,, Los Angeles, CA, United States

This workshop aims to introduce the basic concepts and algorithms for single-cell RNA-seq analysis. It will help participants obtain a better idea of how to use scRNA-seq technology, from considerations in experimental design to data analysis and interpretation. This workshop can serve researchers who are interested in designing an scRNA-seq study or who have acquired […]