Workshop Description (Intermediate Course)
This workshop will cover the basis of Next-Gen Sequencing Library Preparation for Illumina Sequencers. Different Library Preparation Techniques (DNA-seq, ChIP-seq, RNA-seq, Methyl-seq) are explained the first and second day in class. The third day, Quality Control steps of the starting input material and final libraries are performed in the Lab (TapeStation). Purification from Primer Dimers (using AMPure beads) and Library Submission is also performed on the same day. The third day is optional, sign-up is required.
Day 1 - Library Prep 101, focus on DNA
- Theory behind Next Generation Sequencing Library Preparation
- DNA Library Preps (WGS and WES)
- Other Library Preps:
- DNA Methylation
Slides for Day 1- W16_Final_PART1_APRIL2020
Day 2 - Focus on RNA Library Prep
- RNA Library Preps:
- total RNA
- small RNA
- single-cell seq
Slides for Day 2- W16_Final_PART2_APR2020
Day 3 – Session A or session B
(Pellegrini Lab – TLSB, room 4021)
- Quality control of RNA samples
- Quality control of final libraries
- AMPure beads Purification and Size Selection of Sonicated DNA
Slides for Day 3- W16_Final_PART3_APRIL2020
Day 3 will take place in a wet lab. Attendees will need the following prior to the start of class:
- Complete the online Lab Safety Fundamentals Training, found here: http://training.ehs.ucla.edu.
- Bring personal protective equipment.
Dr. Marco Morselli is currently a post-doc in the Lab of Professor Matteo Pellegrini. He received his bachelor and master degrees in Molecular Biotechnology at the University of Parma, Italy. He earned his PhD at UCLA focusing on the crosstalk between histone modifications and DNA methylation. Outside of the lab, Marco enjoys cooking, running, cycling and playing soccer.
It was a super clear lecture. I learned a lot and also was very entertained the whole time. Marco answered questions great and obviously prepared very well for this. Thanks to The Collaboratory for making this happen.
Great workshop! The hands-on portion in the wet lab really increased the intellectual value and added to the experience.
Clear and informative!
Prerequisites: Lab Safety Training
Length: 3 days, 3 hrs per day
Seats Available: TBD
Apr. 20, 21, and 22
1:30 PM to 4:30 PM
REGISTRATION IS CLOSED!