TITLE: “Genome-wide survey of mutations influencing protein abundances in yeast”
ABSTRACT: Our understanding of how protein-level regulation is encoded in the genome and how protein abundances are affected by DNA sequence variation remains sparse. I will discuss our genetic screen based on large-scale pooled base editing that allows us to capture the effects of thousands of mutations on the abundance of individual proteins in yeast. We applied this screen to 11 proteins and gained new insights into the components, scale and connectedness of genetic networks underlying the regulation of protein abundances.
