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QCBio Research Seminar in collaboration with B.I.G. Summer: Harold Pimentel

July 24 @ 2:00 pm - 2:30 pm

TITLE: “Model driven design and analysis of quantitative phenotype screens”

ABSTRACT: Increasingly, CRISPR screens are coupled with flow cytometry (FACS) to sort cells and quantify the

impact of genetic perturbations on a continuous phenotype. While FACS provides much more

quantitative information than simple survival screens, it introduces a number of experimental and

statistical challenges. Furthermore, as experimentalists push these screens in limited primary cells

or in vivo, they lack principled guidelines on how experimental parameters including multiplicity of

infection, guide RNA coverage, or FACS bin cutoffs affect statistical power. We present models for

both experimental design and inference of gene regulation in these screens. We show that

commonly used parameters are far from optimal and screens can be performed with a 20 times

reduction in cells at comparable accuracy. Our inference procedure models biological replicates,

infers the latent protein distribution, and infers experimental parameters. Together these analyses

provide a holistic framework for designing and analyzing highly parallel FACS screens.

Details

Date:
July 24
Time:
2:00 pm - 2:30 pm
Event Category:

Venue

ZOOM
CA United States
Website:
ucla.zoom.us

Organizer

QCBio

Details

Date:
July 24
Time:
2:00 pm - 2:30 pm
Event Category:

Venue

ZOOM
CA United States
Website:
ucla.zoom.us

Organizer

QCBio